Journal article
Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae
HJ Wu, KL Seib, YN Srikhanta, J Edwards, SP Kidd, TL Maguire, A Hamilton, KT Pan, HH Hsiao, CW Yao, SM Grimmond, MA Apicella, AG McEwan, AHJ Wang, MP Jennings
Journal of Proteomics | Published : 2010
Abstract
Neisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defence mechanisms that are dependent on manganese (Mn). We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag [ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype. These studies revealed that there were proteins regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (e.g., pilin, a key adhesin), oxidative stress defence (e.g., superoxide ..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
We thank Dr K H Khoo for his valuable consultation in the field of proteomics This work was supported by Program Grant 284214 from the National Health and Medical Research Council of Australia The Core Facilities for Proteomics Research, Institute of Biological Chemistry, Academia Sinica, is supported by a National Science Council grant (NSC 93-3112B-001-010-Y) and Academia Sinica. Hsing-Ju Wu was supported by the fellowship of Distinguished Postdoctoral Scholar, Institute of Biological Chemistry, Academia Sinica, Taiwan, and also by a CJ Martin NHMRC Career Development award SMG is a recipient of a NHMRC Career Development award and a senior research affiliate of the ARC Special Research Centre for Functional and Applied Genomics. The authors would like to thank NIH and TIGR for the provision of the Neisseria arrays We acknowledge the Gonococcal Genome Sequencing Project supported by USPHS/NIH grant #AI38399, and B A Roe, L Song, S P Lin, X Yuan, S. Clifton, Tom Ducey, Lisa Lewis and D W Dyer at the University of Oklahoma The authors further thank the Cooperative Human Tissue Network (Columbus, Ohio, USA) for providing cervical tissue specimens and the Columbus Children's Research Institute for funding to JLE